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tripan blue solution  (Millipore)


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    Millipore tripan blue solution
    Tripan Blue Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tripan+blue/trypan+blue+solution/pm39175770-147-154-157
    Average 90 stars, based on 1 article reviews
    tripan blue solution - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Electrophysiological Impact of SARS-CoV-2 Envelope Protein in U251 Human Glioblastoma Cells: Possible Implications in Gliomagenesis?
    Article Snippet: Every day for four days, cells underwent trypsinization and were counted with the Bürker chamber after being diluted 1:1 with the vital dye Tripan Blue (Sigma-Aldrich) in order to discard dead cells from the count.

    Article Title: Molecular hierarchy of mammary differentiation yields refined markers of mammary stem cells.
    Article Snippet: Cells were sorted into 96-well plates containing 30 μL 50% (vol/vol) Growth Factor Reduced Matrigel (BD Bioscience) and 0.01% (vol/vol) Tripan Blue (Sigma).



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    The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells (DMSO, PTX-short, ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) Tripan blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.

    Journal: Scientific Reports

    Article Title: Eribulin induces micronuclei and enhances the nuclear localization of cGAS in triple-negative breast cancer cells

    doi: 10.1038/s41598-024-64651-y

    Figure Lengend Snippet: The effects of KD-cGAS on cell proliferation was evaluated. MM231 cells (DMSO, PTX-short, ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI) were used ( a ) Proliferation assay was performed. 24 h after knockdown of cGAS was defined as 0 h, and cell proliferation was evaluated every 12 h. ( b ) Tripan blue stain was used to evaluate the percentage of live and dead cells 24 h after treatment. ( c ) We evaluated the effects of knocking down of cGAS on RAD51 expression. Vinculin was used as loading control. ( d ) Immunofluorescence with RAD51 and γH2Ax was performed. DAPI was used for nuclear staining. The white line is 10 μm. ( e ) We counted the number of nuclear foci in each cell stained with γH2Ax and RAD51 on average. The meaning of the asterisks are as follows: *p < 0.05, **p < 0.01.

    Article Snippet: The cell lysates from MM231 cell lines (DMSO, PTX-short, ERI-short, KD-cGAS-DMSO, KD-cGAS-PTX, KD-cGAS-ERI), which were 24 h after PTX or ERI treatment, were extracted and diluted equally by tripan blue (Nacalai, #20577-34).

    Techniques: Proliferation Assay, Knockdown, Staining, Expressing, Control, Immunofluorescence